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Registros recuperados: 6
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Análisis de imágenes, calidad y maduración de semillas de jiló (Solanum gilo) Agrociencia
Alves,M. Vinicius Prado; Pinho,Édila Vilela de Resende Von; Santos,Heloisa Oliveira dos; Alves,Gustavo Costa Prado; Carvalho,Maria Laene Moreira de; Bustamante,Fernanda de Oliveira.
Resumen El momento de cosecha se correlaciona con la calidad fisiológica de la semilla y puede definirse a través de la germinación y las pruebas de vigor. Los rayos X son una buena técnica para evaluar la calidad de la semilla, ya que pueden reducir la subjetividad del análisis, volviendo el proceso más rápido y más eficiente. Nuestro objetivo fue evaluar el área libre interna de la semilla de jiló (Solanum gilo), a través del análisis de la imagen de rayos X, y relacionar los resultados con la germinación de la semilla en distintas etapas de maduración. Las variables evaluadas fueron: área del objeto, perímetro, desviación estándar, y área de fracción, que representa las áreas libres internas de las semillas. El diseño experimental fue completamente...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jiló; Rayos X; Madurez fisiológica.
Ano: 2018 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952018000200267
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Genomic behavior of hybrid combinations between elephant grass and pearl millet PAB
Leão,Fernando Ferreira; Davide,Lisete Chamma; Campos,José Marcello Salabert de; Pereira,Antonio Vander; Bustamante,Fernanda de Oliveira.
The objective of this work was to evaluate the genomic behavior of hybrid combinations between elephant grass (Pennisetum purpureum) and pearl millet (P. glaucum). Tetraploid (AAA'B) and pentaploid (AA'A'BB) chromosome races resulting from the backcross of the hexaploid hybrid to its parents elephant grass (A'A'BB) and pearl millet (AA) were analyzed as to chromosome number and DNA content. Genotypes of elephant grass, millet, and triploid and hexaploid induced hybrids were compared. Pentaploid and tetraploid genomic combinations showed high level of mixoploidy, in discordance with the expected somatic chromosome set. The pentaploid chromosome number ranged from 20 to 34, and the tetraploid chromosome number from 16 to 28. Chromosome number variation was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; DNA content; Interspecific hybrids; Mixoploidy.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000700006
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Identification of haploid maize by flow cytometry, morphological and molecular markers Ciência e Agrotecnologia
Couto,Evellyn Giselly de Oliveira; Davide,Livia Maria Chamma; Bustamante,Fernanda de Oliveira; Pinho,Renzo Garcia Von; Silva,Tallyta Nayara.
The development of homozygous breeding lines in maize may be accelerated through the use of haploids. Thus, the obtaining and prior identification of haploids generated by the haploid inducer lines is an important factor. The purpose of this study was to identify haploids by flow cytometry and to correlate the nuclear DNA content to the morphological and morphometric traits of the seeds that gave rise to them. In addition, molecular markers were used to confirm the androgenetic nature of the haploid. The seeds obtained were derived from the cross between the inbred line W23 and the commercial hybrid P30F90. Among these seeds, a group was selected, putative haploids, whose embryo was white and the pericarp purplish. This group, consisting of 330 seeds, was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Nuclear DNA content; Androgenetic inheritance; R-navajo; Microsatellites.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000100003
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In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE Ciência e Agrotecnologia
Couto,Evellyn Giselly de Oliveira; Pinho,Édila Vilela de Resende Von; Pinho,Renzo Garcia Von; Veiga,Adriano Delly; Bustamante,Fernanda de Oliveira; Dias,Kaio Olimpio das Graças.
ABSTRACTArtificial chromosome duplication is one of the most important process in the attainment of doubled haploids in maize. This study aimed to evaluate the induction ability of the inducer line KEMS in a tropical climate and test the efficiency of the R1-Navajo marker by flow cytometry to evaluate two chromosome duplication protocols and analyze the development of the doubled haploids in the field. To accomplish this goal, four genotypes (F1 and F2 generations) were crossed with the haploid inducer line KEMS. The seeds obtained were selected using the R1-Navajo marker and subject to two chromosome duplication protocols. Duplication was confirmed using flow cytometry. The percentages of self-fertilized plants after duplication as well as the quantities...
Tipo: Info:eu-repo/semantics/article Palavras-chave: R-Navajo; Colchicine; Induction of maternal haploids; Zea mays.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500435
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Morphology and pollen viability of Lolium multiflorum Lam. Ciência e Agrotecnologia
Nunes,Renata de Castro; Bustamante,Fernanda de Oliveira; Techio,Vânia Helena; Mittelmann,Andréa.
Study and characterization of pollen grains are essential for different areas, especially taxonomy, genetic improvement, phylogeny, and paleobotany. As yet, there are no reports on pollen morphology of genotypes of naturalized Lolium multiflorum Lam., introduced cultivars or breeding populations, diploid or polyploid. Ten genotypes of annual ryegrass (L. multiflorum) were evaluated for the viability of pollen grains using propionic carmine and Alexander's stains, while morphology was assessed by the acetolysis technique. Measures of polar axis (P), equatorial diameter (E), exine thickness, and analysis of pollen grains were obtained by scanning electron microscopy (SEM). All genotypes showed high rate of pollen viability (> 89%) for both stains. There...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scanning electron microscopy; Ryegrass; Acetolysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000200006
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Stability in chromosome number and DNA content in synthetic tetraploids of Lolium multiflorum after two generations of selection Ciência Rural
Pereira,Roselaine Cristina; Santos,Natália de Souza; Bustamante,Fernanda de Oliveira; Mittelmann,Andrea; Techio,Vânia Helena.
ABSTRACT: Chromosome doubling of Italian ryegrass genotypes ( Lolium multiflorum Lam.) adapted to the brazilian edaphoclimatic conditions is an important strategy used by breeders and aims to obtain more vigorous genotypes with better forage quality and disease resistance. The effectiveness of chromosome doubling can be measured by genetic stability and fertility rates of plants over generations. However, a common problem in the polyploidization process is the regeneration of mixoploid plants that have impaired fertility and genetic stability. The objective of this study was to verify if progenies of recently tetraploidized plants remain stable regarding DNA content and chromosome number, over two generations. Progenies of L. multiflorum plants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosomal duplication; Mixoploidy; Italian ryegrass; Plant breeding.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200401
Registros recuperados: 6
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